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KMID : 0378019830260070047
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New Medical Journal
1983 Volume.26 No. 7 p.47 ~ p.53
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The Effect of Warm Ischemia on Kidney in Rats
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고용복/Koh, Yong Bok
김진/김응국/Kim, Eung Kook/Lee, Yong Kak
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Abstract
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Temporary interruption of renal blood flow produce warm ischemic damage to kidney, which is followed by impaired renal function resulted from acute tubular necrosis.
In renal transplantation, there is always possibility of warm ischemic damage to kidney by temporary interruption of blood flow.
It is known that degree of renal damage and recovery from warm ischemia depend on the duration of interruption of blood flow to the kidney.
The author´s experiment was attempted to investigate the correlation between the length, of warm ischemic injury and kidney function, survival and morphological changes of the kidney in rats.
After interruption of all collaterals to the right kidney, the right renal artery and ureter were clamped by tiny bulldog clamps for designed time according to experimental groups.
The renal pelvis and ureter of the left kidney were ligated at the same time and the left kidney was excised out when bulldog clamps on right kidney were released, and used for comparing study.
Experimental rats were devided into 10 minutes (10 rats), 30 minutes (10 rats) and 60 .minutes (10 rats) clamping groups.
The survived rats were sacrified on 15th day after experiment.
Serum creatinine and blood urea nitrogen were checked to monitor periodical renal function. The experimental results were as follows:
1. In survived 10 min., 30 min., and 60 min. groups each of the mean serum creatinine .levels was 0.82 ±0.Olmg/lOOml, 0. 92±0. l0mg/l00ml, and 1.98±0.21mg/10Oml at postoperative .5th day, and each of them decreased to nearly normal level at postoperative 10th and ´15th day. The serum creatinine level of 60 min. group was higher comparing to that of 10 min. and 30 min. group at 5th day (P2. In survived 10 min., 30 min. and 60 min groups, each of the mean blood urea nitrogen levels was 23.81±3.29mg/100m1, 25.10±2.43mg/l00ml and 51.24±7.98mg/l00ml at postope-rative 5th day, each of them were decreased to nearly normal level at postoperative 10th and 15th day. The blood urea nitrogen level of 60 min, group was higher comparing to that of 10 min. and 30min. group at 5th day (P<0.01), but there was no sigificant changes at 10th and 15th day.
3. The mean surum creatinine and blood urea nitrogen levels of expired rats were 2.87± 0.42mg/100ml and 88.32±9.27mg/l00ml, which higher comparing to those of all survived groups at postoperative 5th day (P<0.01), and the microscopic findings of the kidney of expired rats showed severe acute tubular necrosis.
4. In survived 10 min. and 30 min. groups, the microscopic findings were of the kidney mild tubular dilation, regeneration of tubular cell and minimal interstitial fibrosis.
In survived 60 min. group, however, the microscopic findings of the kidney were cicatrical. changes in cortex and medulla, atrophy of tubular cells and infiltration of inflammatory cells.
5. All rats of 10 minutes clamping group survived but 2 rats of 30 mina (20%) and 4 rats of 60 min. (40%) clamping group expired during the experimental period.
This experiments suggested that the tolerable period of warm ischemia on the kidney is within 30 minutes in rats.
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